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( a – d ) Representative histological images of TRAP-positive osteoclasts (arrowheads) within callus tissue of wildtype ( a , b ) and Nlrp3 −/− mice ( c , d ) at 2 ( a , c ) and 5 weeks ( b , d ) after fracture. Scale bars 50 µm. ( e – h ) Representative immunohistochemical images of <t>CD31-positive</t> microvessels (arrowheads) within the callus tissue of wildtype ( e , f ) and Nlrp3 −/− mice ( g , h ) at 2 ( e , g ) and 5 weeks ( f , h ) after fracture. Scale bars: 50 µm. Histological analysis of TRAP-positive osteoclasts/HPF ( i ) and immunohistochemical analysis of CD31-positive microvessels/HPF ( j ) within callus tissue of wildtype (white bars, n = 8) and Nlrp3 −/− mice (black bars, n = 9–10) at 2 and 5 weeks after fracture (mean ± SEM). * p < 0.05 vs. wildtype.
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( a – d ) Representative histological images of TRAP-positive osteoclasts (arrowheads) within callus tissue of wildtype ( a , b ) and Nlrp3 −/− mice ( c , d ) at 2 ( a , c ) and 5 weeks ( b , d ) after fracture. Scale bars 50 µm. ( e – h ) Representative immunohistochemical images of <t>CD31-positive</t> microvessels (arrowheads) within the callus tissue of wildtype ( e , f ) and Nlrp3 −/− mice ( g , h ) at 2 ( e , g ) and 5 weeks ( f , h ) after fracture. Scale bars: 50 µm. Histological analysis of TRAP-positive osteoclasts/HPF ( i ) and immunohistochemical analysis of CD31-positive microvessels/HPF ( j ) within callus tissue of wildtype (white bars, n = 8) and Nlrp3 −/− mice (black bars, n = 9–10) at 2 and 5 weeks after fracture (mean ± SEM). * p < 0.05 vs. wildtype.
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( a – d ) Representative histological images of TRAP-positive osteoclasts (arrowheads) within callus tissue of wildtype ( a , b ) and Nlrp3 −/− mice ( c , d ) at 2 ( a , c ) and 5 weeks ( b , d ) after fracture. Scale bars 50 µm. ( e – h ) Representative immunohistochemical images of <t>CD31-positive</t> microvessels (arrowheads) within the callus tissue of wildtype ( e , f ) and Nlrp3 −/− mice ( g , h ) at 2 ( e , g ) and 5 weeks ( f , h ) after fracture. Scale bars: 50 µm. Histological analysis of TRAP-positive osteoclasts/HPF ( i ) and immunohistochemical analysis of CD31-positive microvessels/HPF ( j ) within callus tissue of wildtype (white bars, n = 8) and Nlrp3 −/− mice (black bars, n = 9–10) at 2 and 5 weeks after fracture (mean ± SEM). * p < 0.05 vs. wildtype.
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( a – d ) Representative histological images of TRAP-positive osteoclasts (arrowheads) within callus tissue of wildtype ( a , b ) and Nlrp3 −/− mice ( c , d ) at 2 ( a , c ) and 5 weeks ( b , d ) after fracture. Scale bars 50 µm. ( e – h ) Representative immunohistochemical images of <t>CD31-positive</t> microvessels (arrowheads) within the callus tissue of wildtype ( e , f ) and Nlrp3 −/− mice ( g , h ) at 2 ( e , g ) and 5 weeks ( f , h ) after fracture. Scale bars: 50 µm. Histological analysis of TRAP-positive osteoclasts/HPF ( i ) and immunohistochemical analysis of CD31-positive microvessels/HPF ( j ) within callus tissue of wildtype (white bars, n = 8) and Nlrp3 −/− mice (black bars, n = 9–10) at 2 and 5 weeks after fracture (mean ± SEM). * p < 0.05 vs. wildtype.
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Fig. 4. Subcutaneous adipose tissue promoted vascularization and cell proliferation in expanded skin by means of paracrine growth factors. (Above) Immunohistochemical staining for CD31 and <t>PCNA</t> to mark ves- sels and <t>proliferating</t> cells. (Center) mRNA expression levels of EGF, bFGF, and VEGF in skin samples from the control group and the AT group (AT-Skin), and adipose tissue from the AT group (AT-Adipose). (Below, right) Immunoblot analysis of the expression of bFGF, EGF, and VEGF in skin samples from the control group and the AT group, and adipose tissue from the AT group. Scale bar = 100 µm; *P < 0.05; ***P < 0.001.
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( a – d ) Representative histological images of TRAP-positive osteoclasts (arrowheads) within callus tissue of wildtype ( a , b ) and Nlrp3 −/− mice ( c , d ) at 2 ( a , c ) and 5 weeks ( b , d ) after fracture. Scale bars 50 µm. ( e – h ) Representative immunohistochemical images of CD31-positive microvessels (arrowheads) within the callus tissue of wildtype ( e , f ) and Nlrp3 −/− mice ( g , h ) at 2 ( e , g ) and 5 weeks ( f , h ) after fracture. Scale bars: 50 µm. Histological analysis of TRAP-positive osteoclasts/HPF ( i ) and immunohistochemical analysis of CD31-positive microvessels/HPF ( j ) within callus tissue of wildtype (white bars, n = 8) and Nlrp3 −/− mice (black bars, n = 9–10) at 2 and 5 weeks after fracture (mean ± SEM). * p < 0.05 vs. wildtype.

Journal: International Journal of Molecular Sciences

Article Title: Nlrp3 Deficiency Does Not Substantially Affect Femoral Fracture Healing in Mice

doi: 10.3390/ijms252111788

Figure Lengend Snippet: ( a – d ) Representative histological images of TRAP-positive osteoclasts (arrowheads) within callus tissue of wildtype ( a , b ) and Nlrp3 −/− mice ( c , d ) at 2 ( a , c ) and 5 weeks ( b , d ) after fracture. Scale bars 50 µm. ( e – h ) Representative immunohistochemical images of CD31-positive microvessels (arrowheads) within the callus tissue of wildtype ( e , f ) and Nlrp3 −/− mice ( g , h ) at 2 ( e , g ) and 5 weeks ( f , h ) after fracture. Scale bars: 50 µm. Histological analysis of TRAP-positive osteoclasts/HPF ( i ) and immunohistochemical analysis of CD31-positive microvessels/HPF ( j ) within callus tissue of wildtype (white bars, n = 8) and Nlrp3 −/− mice (black bars, n = 9–10) at 2 and 5 weeks after fracture (mean ± SEM). * p < 0.05 vs. wildtype.

Article Snippet: For the immunohistochemical detection of microvessels, the sections were stained with a monoclonal rat anti-mouse antibody against endothelial cell marker CD31 (1:100; Dianova, Hamburg, Germany).

Techniques: Immunohistochemical staining

Fig. 4. Subcutaneous adipose tissue promoted vascularization and cell proliferation in expanded skin by means of paracrine growth factors. (Above) Immunohistochemical staining for CD31 and PCNA to mark ves- sels and proliferating cells. (Center) mRNA expression levels of EGF, bFGF, and VEGF in skin samples from the control group and the AT group (AT-Skin), and adipose tissue from the AT group (AT-Adipose). (Below, right) Immunoblot analysis of the expression of bFGF, EGF, and VEGF in skin samples from the control group and the AT group, and adipose tissue from the AT group. Scale bar = 100 µm; *P < 0.05; ***P < 0.001.

Journal: Plastic & Reconstructive Surgery

Article Title: Tracing the Change and Contribution of Subcutaneous Adipose to Skin Expansion Using a Luciferase-Transgenic Fat Transplantation Model

doi: 10.1097/prs.0000000000010753

Figure Lengend Snippet: Fig. 4. Subcutaneous adipose tissue promoted vascularization and cell proliferation in expanded skin by means of paracrine growth factors. (Above) Immunohistochemical staining for CD31 and PCNA to mark ves- sels and proliferating cells. (Center) mRNA expression levels of EGF, bFGF, and VEGF in skin samples from the control group and the AT group (AT-Skin), and adipose tissue from the AT group (AT-Adipose). (Below, right) Immunoblot analysis of the expression of bFGF, EGF, and VEGF in skin samples from the control group and the AT group, and adipose tissue from the AT group. Scale bar = 100 µm; *P < 0.05; ***P < 0.001.

Article Snippet: To detect the vessels and proliferating cells (early G1 and S phases) in expanded skin, rabbit anti-rat CD31 (BM2966; Boster, Wuhan, People’s Republic of China) and mouse anti-rat proliferating cell nuclear antigen (PCNA) (BM0104; Boster) antibodies were applied.

Techniques: Immunohistochemical staining, Staining, Expressing, Control, Western Blot